Coding

Part:BBa_K1129001:Design

Designed by: UBC iGEM 2013   Group: iGEM13_British_Columbia   (2013-08-30)


Feruloyl-CoA-Synthetase (FCS) from Pseudomonas putida KT2440


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 43
    Illegal XhoI site found at 1153
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 30
    Illegal NgoMIV site found at 294
    Illegal NgoMIV site found at 772
    Illegal NgoMIV site found at 1027
    Illegal NgoMIV site found at 1090
    Illegal NgoMIV site found at 1107
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 802


Design Notes

Illegal cut sites removed


Source

Pseudomonas putida KT2440

References